Seoul asan hospital Jae-Min Kim conducted a study on receiving growth hormone (GH) treatment changes the serum protein mass spectrometry.The
Seoul asan hospital Jae-Min Kim conducted a study on receiving growth hormone (GH) treatment changes the serum protein mass spectrometry.
The study included 6 children with idiopathic short stature. The researchers collected the serum from patients before and after 3 months of treatment with GH, and removed the expression of the most abundant proteins in each serum sample by multiple affinity removal column (MARC) (). Then the MARC treated serum was analyzed by two-dimensional gel electrophoresis, and the results were analyzed by Melanie 7. The results of electrophoresis showed that the band value of GH was 2 times higher than that before treatment, and the protein chip fingerprint time of flight mass spectrometry (MALDI-TOF) was used.
The results showed that the expression of GH protein was different in different patients, and the degree of elevation was not very obvious. There are 19 kinds of proteins which are expressed in GH after treatment, including complement 3, haptoglobin CRA-b, myosin, alpha -2- giant globulin precursor, keratin 7/10, actin and tubulin. It is difficult to speculate on the clinical significance of these proteins, but after treatment with GH, serum levels of cytoskeletal proteins and acute phase response proteins increased.
The researchers believe that after GH treatment, serum protein expression profile has changed, and this change may be identified as a biomarker for the treatment of patients with GH response.
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